Effects Of Genetic Mutations On Human Evolution - 2063 Words

Alek Petuskey Mrs. Yang Period 5 April 29, 2015 Polycythemia and Lip Augmentations Affect on Human Evolution Mutations and errors in sexual reproduction allow for variation within a population. These variations can eventually lead to the complex process of evolution in a species. The genetically derived mutation Polycythemia and the environmentally derived mutation lip augmentations, affect human evolution in a beneficial and detrimental way. Evolution is change in the aspects of a certain species over a period of time. This is caused by mutations which are variations or differences in the genome. Both genetic and environmental mutations affect the evolution of life in a negative and positive way. Although genetic mutations are heritable some environmental mutations are inheritable because they are not encoded in the genome. Although theories of evolution were published prior, the first person to discover and apply natural selection to evolution was Charles Darwin. Previous scientists such as Lamarck thought evolution occurred b y the passing of acquired traits. Darwin s theory was different because he theorized that evolution occurred with the passing down of heritable traits that were not acquired. Darwin stated that a species â€Å"fitness or ability to survive and reproduce determined if it’s genes were passed on. Organisms within a species with a desirable trait that favored them to survive were more likely to pass on their genes than an organism who didn t haveShow MoreRelatedEssay about Evolution1502 Words   |  7 Pagesto humans, there are many traits that have been selected and adapted for throughout their evolutionary history giving them the characteristics that they have today. In this paper I will discuss some parts of the human body, which have been found to be selected for by the evolutionary mechanisms of natural selection, adaptation and mutation. Natural selection, adaptation, and mutations are three components of the evolutionary process, each one having either positive or negative effects onRead MoreEvolution Is The Gradual Change Of Genotype And Phenotype Of Organisms1415 Words   |  6 PagesEvolution is the gradual change of genotype and phenotype of organisms over time in response to selection pressures. This enables organisms to become better equipped to exploit their environment. Random processes are governed by the laws of probability and cannot be forecast except for a certain degree of directionality. Deterministic processes however, are predictable and tend towards a repeated outcome. It is necessary to consider the true role of stochasticity in evolution, first in the frameRead MoreEvolution By Natural Selection Is A Theory Created By Charles Darwin1415 Words   |  6 PagesHewitt 1 Kyle Hewitt Period 3 5/3/15 A World Full of Mutations We live in a world full of mutations. These mutations are sources of variation, and are essential to our survival. As generations of organisms progress, we develop mutations that help us to adapt to the world around us. This is called evolution. Evolution by natural selection is a theory created by Charles Darwin in which he states that organisms evolve over time and develop certain characteristics, and the beneficial characteristicsRead MoreAp Bio Chapter 231661 Words   |  7 Pages\ Chapter 23 Reading guide 1. what is the smallest unit of evolution and why is this important to understand? The population is the smallest unit of evolution . This is important to understand because it keeps clear what is evolving. 2. Define the following terms: a. Microevolution: evolutionary change below the species level; change in the genetic makeup of a population from generation to generation. It is evolutionary change on its smallest scale b. Population: a localized groupRead MoreOrigin Of Life On Earth And How Biological Populations Have Changed And Developed Over Successive Generations1635 Words   |  7 Pages The theory of evolution discusses, with considerable proof, the history of life on earth and how biological populations have changed and developed over successive generations. Despite the abundance of evidence for evolution, both scientific and physical, some still doubt the validity of this theory. However, once compared with the alternative theory of creation, it is apparent that evolution is the most probable explanation for the origin of life on earth, to date. This essay will explain a handfulRead MoreContagion : A Perspective Of A Worldwide Pandemic997 Words   |  4 PagesThe movie Contagion gives a perspective of a worldwide pandemic as it begins and spreads throughout the world while displaying signs of evolution. The movie begins with a woman, Beth, traveling home from a business trip in Hong Kong. Beth becomes sick with what she thinks is the flu. However, she suffers from a seizure and dies suddenly from an unknown cause. Her son along with several other cases around the world suddenly die after having similar symptoms Her husband Mitch discovers that he isRead MoreTheory of Evolution: The Evolution of Hox Cluster Essay808 Words   |  4 Pagesnatural selection was how evolution took place, yet you did not know how this mechanism was actually involved in evolution. Well you were right and now we know how natural selection plays a part in evolution. Natural selection does play a big part but there are others mechanisms that help shape how an organism evolves. These mechanisms include migration, isolation, genetic drift, and mutations. A specific new field in biology has developed, in which explicitly looks at mutations and how it causes theRead MoreEvolution And Evolution Of Evolution1029 Words   |  5 Pagesplant over billions of years was the establishment and evolution of life as we understand today. In the sciences, evolution is a widely-accepted theory that posits the development, alteration, and otherwise change of a population of species. The evolution of a group of organisms is often mistakenly assumed to always be positive (i.e. the misconception that evolution is always a progression towards a â€Å"better† organisms). Given that evolution is a process that generally guides groups of organismsRead MoreEvolution And Evolution Of Evolution1137 Words   |  5 Pagesasked about the definition of evolution, they might say some jumble thoughts about Darwin’s theories and monkeys evolving into humans. That may be all they can say about it, but they know there’s more to evolution than that. This is probably all they can say with any authority about what they know. In secondary school, teachers will often awkwardly past over the subject without going into much depth; this maybe because of past controversies about the teaching of evolution in secondary school. But asRead MoreThe Proces of Evolution653 Words   |  3 PagesWithout evolution, and the constant ever changing environment, the complexity of living organisms would not be as it is. Evolution is defined as a process that results in heritable changes in a population spread over many generations (8).Scientists believe in the theory of evolution. This belief is based on scientific evidence that corroborates the theory of evolution. In Figure 1 the pictures of the skulls depict the sequence of the evolution of Homo-sapiens. As the figure shows, man has evolved

Lady Macbeth By William Shakespeare - 1520 Words

Lady Macbeth was not evil she was misunderstood Claim thou art, and Cawdor and shalt be what thou art promised. (Quote) In many of his plays, William Shakespeare portrays women as more virtuous than men. Lady Macbeth, strong ambitious women who’s confidence, faithful and capable of almost anything. For example, in endless love, we notice the endless love they both share for each other, where fate still brought them back together even after all the influences affecting jade’s life, the overpowering of her father.More similarly, in The Winter s Tale, we witness Hermione patiently enduring her miserable plight at the hands of the raging Leontes. Furthermore, we notice that very few of Shakespeare s villains are female. in quite a few of his written pieces, Shakespeare seems to support the view that women are by nature tender, loving, and incapable of the evils which men often commit. Lady Macbeth strongly stood against tradition, portraying such masculine characteristics. Lady Macbeth was not evil, she was misunderstood, she showed endless love for her spineless husband, she mourned the loss of her young child. Lady Macbeth also showed quite ambition, traditionally this was not accepted within the community Reading the play of Macbeth, Macbeth appears to run counter to this common image of women, for in this tragedy, we see a wiliness Lady Macbeth planning to kill king Duncan Her brutality in pressuring Macbeth to carry out the murder leads us to believe that LadyShow MoreRelatedMacbeth And Lady Macbeth By William Shakespeare1343 Words   |  6 PagesIn William Shakespeare’s epic tragedy, Macbeth, the concept of the every blurry line between good and evil is shown through the two main characters, Macbeth and Lady Macbeth. This husband and wife duo begins the play as polar opposites. Lady Macbeth’s maliciousness and drive is clear to the audience the first time she steps on stage in Act One Scene Five. She continues on with these same characteristics, yet in the middle of the drama, there is a distinct change in her attitude and overall role asRead MoreThe Ambition of Lady Macbeth in Macbeth by William Shakespeare894 Words   |  4 PagesThe Ambition of Lady Macbeth in Macbeth by William Shakespeare Timothy Leary once said, Women who seek to be equal with men lack ambition (Peters 175). But is this true in the case of the classic play Macbeth? In Macbeth it seems to be, that Macbeth the protagonist of the play is influence by Lady Macbeths ambition. Could this be an exception or was Lady Macbeth lying when she ask to be equal to a man so she could commit the murder (1.5.33.45-61). To understand one must look deeply into theRead MoreMacbeth And Lady Macbeth By William Shakespeare959 Words   |  4 Pagesoccasion. This is clearly illustrated through Duncan s murder in the story Macbeth between the two characters Macbeth and Lady Macbeth. Their responses are shown through their initial reaction to the thought of murdering Duncan, immediately prior to the murder of Duncan, and immediately after the murder of Duncan. Duncan was a kind and good man, and if Macbeth were to kill Duncan, then he would be the bad guy. Macbeth found that there was no reason to kill Duncan other than only for personal gainRead MoreLady Macbeth by William Shakespeare824 Words   |  3 PagesIn Shakespeare play, Macbeth, Lady Macbeth’s character progresses in an interesting manner. Lady Macbeth is made to act as an incentive to Macbeths immoral actions. Even though Macbeth is generally the person to have a final say before killing someone, Lady Macbeth plays the role of his â€Å"sidekick†. She mocks her husband if he worries over a sinful deed (which usually she instructs him to do), saying he would be less of a man if he does not follow through with their plan (I. vii. 56-57). She givesRead MoreLady Macbeth By William Shakespeare1075 Words   |  5 Pages In Shakespeare’s Macbeth, the motif of night is aggressive like the murders they plot and commit. Night dominates day throughout the play since they commit murders which disrupt the natural order of the world. Macbeth â€Å"plays God† by taking someone else’s life and therefore they challenge the Elizabethan order of the world. Acts of chaos, like earthquakes and supernatural events, break loose. Macbeth and Lady Macbeth think the night will cover their treacherous deeds. At first, the idea of murderingRead MoreLady Macbeth, By William Shakespeare Essay1674 Words   |  7 PagesThe obvious reading of this passage would be of Lady Macbeth as domineering and manipulative in pursuit of her own goals. But that reading discounts the loyalty and dedication she has shown to Macbeth and his success. By encouraging violence through questioning his manhood, masculinity and violence become inextricably linked. This connection essentially defines masculinity as violence wit hin the world of Macbeth. The relationship between power, violence, and masculinity alienate women and power andRead MoreLady Macbeth By William Shakespeare1280 Words   |  6 PagesIntroduction Lady Macbeth is a big character one of the most complex characters within the play. She is a mixture of contradictions she is strong, ambitious, ruthless, weak and manipulative to name a few. In the beginning of the play Lady Macbeth is strong, ruthless and ambitious, in Act1 Sc.3. Lady Macbeth s more ambitious urges lead Macbeth to the path of crime. She is cold and nasty; she shows no emotion when she believes both her and Macbeth’s dreams are under threat. Lady Macbeth believes herRead MoreLady Macbeth By William Shakespeare1536 Words   |  7 PagesIn the play Macbeth, the secondary characters such as Lady Macbeth, the witches, and Macduff influence the theme and plot. One of the most influential female characters in the tragedy is Lady Macbeth; she has a significant impact on Macbeth s life. Throughout Shakespeare’s time’s, women were not treated with respect; they were regarded as their husbands slaves. However, this is not the case in Macbeth’s marriageRead MoreLady Macbeth By William Shakespeare1299 Words   |  6 PagesLady Macbeth was not evil she was misunderstood Claim thou art, and Cawdor and shalt be what thou art promised. (Quote) In many of Shakespeare’s plays, he portrays women more honourable than men. Lady Macbeth is one of the main antagonist, she’s a strong ambitious women who’s strives with confidence and capable of almost anything. For example in the winter tale, the main antagonist Hermonie, we can interpret that Hermione patiently despairing the dilemma within the hands of the dominant LeontesRead MoreLady Macbeth By William Shakespeare1393 Words   |  6 PagesThe women figure is often perceived as passionate and loving but it was used as a sign of trouble, jealousy, and evilness in old literature pieces such as the Homer’s Odyssey and Shakespeare’s Macbeth, where the storylines couldn’t take places without the intervention of a female character. Both pieces of literature share the appearance of intriguing actions of a female character more than once in the plot. In the Odyssey, we have the female appearance that make an impact to the story three time:

The initiative of One Belt One Road-Free-Samples-Myassignment

Question: Discuss about the One Belt One Road (OBOR) that was declared by the Chinese President, Xi JingPing during the year 2013. Answer: Introduction The current study elucidates in detail about the initiative of One Belt One Road (OBOR) that was declared by the Chinese President, Xi JingPing during the year 2013. Essentially, this scheme was necessarily a crucial foreign strategy for China as this scheme had the intention to promote monetary assistance amongst countries together with the belt as well as road way. The OBOR scheme can hereby be considered as an outline for generation of superior cooperation and assimilation by means of development of an infrastructure network. The current study also presents the capabilities of Singapore companies to grab this opportunity, probable challenges encountered along with the government support and assistance delivered. Capabilities of Singapore companies to capture this opportunity The exclusive position of the nation Singapore under the scheme One Belt, One Road can be evaluated for comprehending the potential of the country to capture this opportunity. As per the framework and structure of Vision and Actions, particularly South East Asia can be considered to be a significant area of the scheme and reinforcing the framework of the nations together with the course is an important priority (The Straits Times 2017). Connectivity As far as connectivity is concerned, there are nearly 200 shipping streak that join the nation Singapore to more than 600 ports in over and above 120 countries right round the globe. Essentially, Chinese enterprises have necessarily observed about impact of the city and their influence on businesses and trade in Southeast Asia in recent years and China has enhanced the entire ante in the deal in Singaporean outline (Winter 2016). The transportation infrastructure includes highway, roads as well as railways that can be considered as one of the obstacles that that these come across in attaining the complete economic prospective. The scheme makes every effort to endorse capital as well as outlays for technological advancements by mainly China into particularly linkages such as ports, courses and ways of transport as well as other infrastructure. This in turn can help in enhancement of the processes of resource circulation, assimilation of the market and permit improved facilitation of b oth business and ventures in Southeast Asia (The Straits Times 2017). Financial services and trade Singapore can be considered to be principal financial centre and the best hub or centre for particularly commodities as well as oil business in primarily Asia that can help in acquirement and distribution of equity and debt capital. This can help in facilitating investment on other areas of the region counting the ASEAN nations. There is also a crucial association centre for the country China, markets of Asia-Pacific and Asia (Yuan et al. 2016). In essence, consistent with the position, the country Singapore can be considered to be strong and well-regulated and clear capital markets. This has important concentration of speciality in the areas of banking as well as financial services, finances along with investment management along with private equity (Yuan et al. 2016). Singapore is a focal point for foreign venture that has a well synchronized legal framework with very lower corruption levels. As such, foreign financiers do not necessarily enter into definite joint course of action and has more than 70 monetary as well as tax treaties with other countries, and proved to be a lucrative spot for multi-state business concerns Economic Policies selected by the government: Rationale for selection of economic policy The initiative can be observed as an attempt to augment trade as well as political associations amongst nations such as China, Asia together with Europe. Also, this One Belt One Road strategy is anticipated to augment growth by exporting huge capital, innovative and advanced technology and capacity internationally (Winter 2016). The adoption of this policy can thereby be said to be effective as the implementation of the development plans together with the trade route might help in improving overall connectivity in five specific areas. The identified areas include policy, trade, people as well as infrastructure. Therefore, with the adoption of the OBOR initiative, the nation can adopt the strategy of augmentation of trade as well as facilitation dimensions, development of requisite infrastructure (Tsui et al. 2017). Economic conjecture that can help in explaining the adoption of the OBOR: The economic initiative of One Belt One Road (OBOR) is expected to enhance the infrastructure and serve as a blueprint or a roadmap for growing worldwide influence. As rightly indicated by Mankiw (2014), economic potential of the initiative is said to enhance the investment scenario in Singapore and affect the overall aggregate demand. Mankiw (2014) mentions that aggregate demand is necessarily the entire demand for particularly goods as well as services manufactured within a specific economy over a specific time period. The equation for aggregate demand (AD) is as presented below: Aggregate Demand (AD) = Consumer Expenditure (C) + (Gross Capital Investment- Investment Expenditure on diverse capital goods)+Government Spending+(Exports-Imports of goods and services) The increase in investment can lead to an increase in aggregate demand. The increase in investment directs the way towards lower rates of interest and makes borrowing for the purpose of investment cheaper. In addition to this, this can also lead to increase in overall confidence in the overall economic outlook, enhanced technology, augmented economic growth in order to satisfy augmented demand of corporations to enhance capability. Mankiw (2014) asserts that investment implies expenses on capital spending. Therefore, investment can be regarded as an element of Aggregate Demand (AD). Nevertheless, in case if the economy is close enough to full capacity, then increase in aggregate demand can direct the way towards inflation and not an enhancement in real GDP. Nevertheless, there are other facets that affect the aggregate demand besides investment. For instance, the in case if there is a decrease in consumer spending or else a decline in the exports, then this leads to an increase in investment that might not perhaps enhance the aggregate demand. In addition to this, the economy of Singapore essentially has scarce capability and an increase in investment can create multiplier effect. The initial increase in investment enhances economic growth, however, in case if the firm acquires more number of sales and profit. Again, they are eager to put into this in further amount of investment (Tsui et al. 2017). In addition to this, households that again acquire employment from specific investment have more earnings to spend. For example, an investment worth USD 2 billion can lead to final increase in real gross domestic product by around USD 3 billion. In this case, there is said to be a multiplier effect of approximately 1.5(Borio 2014). In essence, investment undertaken by the nation particularly for development of advanced infrastructure as well as capital can enhance the overall productivity together with the productivity capability (Heijdra 2017). The above figure hereby reflects that investment can hereby direct the way towards higher real gross domestic product and without overall increase in price (inflation). The adoption of the OBOR initiative reflects government investment that in turn can enhance overall industrial capacity. However, in general there is also misplaced government expenditure that can ideally be inefficient and fail to attain productivity in the nation. Also, there are nations that might have various supply limits specifically in case of public goods such as infrastructure, roadways as well as bridges (Agnor and Montiel 2015). Possible challenges ahead encountered by Singapore companies Politic risks: As rightly put forward by Mankiw (2014), with enhancement of diverse cross border actions, there might be an increase in the political uncertainty, associating to the trade embargos, framework, impediment as well as corruption particularly amongst developing countries. Nations together with the belt and Road would have to deliberately operate together for preventing this kind of risks and arrange a well structured insurance program. Financing the projects: National Development Minister, which is Lawrence Wong reported that Singapore can contribute towards policy of financing projects and deliver proficiency in infrastructure planning. OBOR proved to be an appropriate platform for national integration that in turn can deliver a tremendous boost trade, connectivity and infrastructure development. In essence, financial institutions namely the Asian Infrastructure Investment Bank as well as BRICS New Development Bank are instituted to enhance the overall extent of multilateral financial assistance. Corruption and poor governance: As correctly mentioned by van Vliet (2015), government failures, starting from corruption to ineffective execution of different reforms also pose a severe threat as social as well as political tensions does. In essence, the challenges might possibly be very complex; nevertheless, the formula for navigating the challenges can prove to be very simple. Risk of exchange rates: Yuan appreciation can essentially systematically reduce the overall acquisition cost of certain foreign assets and enhance the profitability of corporations foreign branches as well as subsidiaries. Again, exchange rate volatility in OBOR nations is not very much sensitive to volatility in the rate of exchange. In essence, this specific risk group enumerates the devaluation risk against the reference currency (Mankiw 2014). Particularly, currency risk rating is mainly informed by specific scores for a blend of cyclical, political, strategy as well as structural variables. Influence of the maritime lanes: The involvement of Singapore in particularly Chongqing Connectivity Initiative (CCI) can permit the republic to acquire advantage from the Belt and Road programme. The Maritime Silk Road can necessarily complement the entire Silk Road Economic Belt, concentrating on using sea routes as well as Chinese coastal ports to necessarily link China with necessarily Europe through South China Sea, Indian Ocean, and South Pacific Ocean via the South China Sea. Government Support and Assistance The government intends to utilize OBOR as a specific platform that can help in addressing the nations surplus capacity (Aoyama 2016). The government have the need to regulate as well as coordinate effectively, whilst operating to make certain that competition is fair as well as constructive. Monitoring financing condition (public or private sector) The government of the nation monitor both the domestic as well as worldwide trends and thereby undertake different research studies that have a bearing on different economic policies and management. Thus, the governing bodies monitor the entire project financing structure for building, operating as well as transferring a project. Also, the governing body can examine the financing mechanisms that include process of government financing, financing by private operators and project finance (YANG et al. 2015) Affordable loan financing The loan financing refers to a specific mechanism of financing in which a corporation can accept a loan and provide the same to make repayments of the loan. Implementing skills training/retraining program Gaps can be noticed in areas of knowledge as well as skills that can be related to the implementation of OBOR. The One Belt One Road initiative necessarily has worldwide strategic significance and involves economic growth, trade alteration, transportation and generation of political flexibility (Yeixa and Liang 2017). Thus, it can be hereby mentioned that the government can focus on making investments on training of different workers and providing adequate education. Therefore, training the labour force, provide education as well as other associated skills will essentially enhance the quality of the corridor. Strengthening RMB business hub Strengthening RMB indicates towards the fact that the currency comparatively has higher power in comparison to different other assets/reserves (Huang 2016). In essence, a strong RMB can provide China the advantages related to the international currency such as dollar else wise Euro. As such, the positive trend of strengthening RMB can lead to emergence of a huge market of Chinese consumers as the purchasing power of the nation increases. Development of this market can call for the need of broad reforms and better governance. Both Singapore and China can strengthen cross-border flows of Renminbi (RMB) and collaborate on capital market connectivity that in turn can create huge opportunity for businesses interested in investing. Conclusion The above study has helped in gaining comprehensive understanding regarding initiative of One Belt One Road (OBOR) and the economic concepts and theories associated to the same. In essence, this study has provided an insight regarding the capabilities that the Singapore companies have to realize the advantages of this initiative along with the challenges that are encountered by them. Moving further, the study also presents recommendations regarding the government support that can boost the initiative. References Agnor, P.R. and Montiel, P.J., 2015.Development macroeconomics. Princeton university press. Available at: https://books.google.co.in/books?hl=enlr=id=d6DGBgAAQBAJoi=fndpg=PP1dq=Ag%C3%A9nor,+P.R.+and+Montiel,+P.J.,+2015.+Development+macroeconomics.+Princeton+university+press.ots=XaJcRgkykfsig=AFJj9boUX6IfgON3CH5t8ZfsauM#v=onepageqf=false [Accessed 20 Dec. 2017]. Aoyama, R., 2016. One Belt, One Road: China's New Global Strategy.Journal of Contemporary East Asia Studies,5(2), pp.3-22. Available at: https://www.tandfonline.com/doi/abs/10.1080/24761028.2016.11869094 [Accessed 20 Dec. 2017]. Borio, C., 2014. The financial cycle and macroeconomics: What have we learnt?.Journal of Banking Finance,45, pp.182-198. Available at: https://www.sciencedirect.com/science/article/pii/S0378426613003063 [Accessed 20 Dec. 2017]. Heijdra, B.J., 2017.Foundations of modern macroeconomics. Oxford university press. Available at: https://books.google.co.in/books?hl=enlr=id=14wtDwAAQBAJoi=fndpg=PP1dq=Heijdra,+B.J.,+2017.+Foundations+of+modern+macroeconomics.+Oxford+university+press.ots=Tykl5OCVZysig=ioSDApeNmkphG2LHYxfs3IBVc9E#v=onepageqf=false [Accessed 20 Dec. 2017]. Huang, Y., 2016. Understanding China's Belt Road Initiative: Motivation, framework and assessment.China Economic Review,40, pp.314-321. Available at: https://www.sciencedirect.com/science/article/pii/S1043951X16300785 [Accessed 20 Dec. 2017]. Liu, H.Y., Tang, Y.K., Chen, X.L. and Poznanska, J., 2017. The Determinants of Chinese Outward FDI in Countries Along One Belt One Road.Emerging Markets Finance and Trade,53(6), pp.1374-1387. Available at: https://www.tandfonline.com/doi/abs/10.1080/1540496X.2017.1295843 [Accessed 20 Dec. 2017]. Mankiw, N.G., 2014.Principles of macroeconomics. Cengage Learning. Available at: https://books.google.co.in/books?hl=enlr=id=AObKAgAAQBAJoi=fndpg=PR6dq=Mankiw,+N.G.,+2014.+Principles+of+macroeconomics.+Cengage+Learning.ots=-NactPuSWcsig=X9hdYfnzcx2c4VmBZ9yqb3X_UlA#v=onepageqf=false [Accessed 20 Dec. 2017]. Pitlo III, L.B., 2015. Chinas One Belt, One Road to Where?.The Diplomat,17. Available at: https://thediplomat.com/2015/02/chinas-one-belt-one-road-to-where/[Accessed 20 Dec. 2017]. The Straits Times. 2017.How Singapore can gain from One Belt, One Road initiative. [online] Available at: https://www.straitstimes.com/opinion/how-spore-can-gain-from-one-belt-one-road-initiative [Accessed 20 Dec. 2017]. Tsui, S., Wong, E., CHI, L. and Tiejun, W., 2017. One Belt, One Road.monthlyreview. org/2017/01/01/one-belt-one-road. Available at: https://www.researchgate.net/profile/Vasily_Erokhin/publication/319109329_Trade_between_China_and_the_Countries_of_Central_and_North_Asia_Dynamics_Structure_and_Major_Tendencies/links/5991adfc0f7e9b433f3e8947/Trade-between-China-and-the-Countries-of-Central-and-North-Asia-Dynamics-Structure-and-Major-Tendencies.pdf [Accessed 20 Dec. 2017]. van Vliet, J., 2015. ECON 203-01, 02, 03: Principles of Economics-Macro. Available at: https://digitalcollections.dordt.edu/spring2015/140/ [Accessed 20 Dec. 2017]. Winter, T., 2016. One belt, one road, one heritage: Cultural diplomacy and the Silk Road.The Diplomat,29. Available at: https://s3.amazonaws.com/academia.edu.documents/44318360/One_Belt__One_Road__One_Heritage.pdf?AWSAccessKeyId=AKIAIWOWYYGZ2Y53UL3AExpires=1513744701Signature=GP7AEZTuK6rvxT%2BYsPyrm%2FMEYUU%3Dresponse-content-disposition=inline%3B%20filename%3DOne_Belt_One_Road_One_Heritage_Cultural.pdf [Accessed 20 Dec. 2017]. YANG, B., CHEN, Y. and LV, X., 2015. Spatial response to the" one belt and one road" strategy. InUrban Planning Forum(Vol. 2, pp. 6-23). Available at: https://en.cnki.com.cn/Article_en/CJFDTotal-CXGH201502004.htm [Accessed 20 Dec. 2017]. Yeixa, S. and Liang, Z., 2017. The Three Financial Security Issues in One Belt and One Road Initiatives Strategy Implementation.DEStech Transactions on Social Science, Education and Human Science, (eemt). Available at: https://dpi-proceedings.com/index.php/dtssehs/article/view/14433 [Accessed 20 Dec. 2017]. Yuan, C.Q., Su, C.J. and Wang, Y., 2016. Application and Development of One Belt and One Road Economic Plants (Africa).Chinese Wild Plant Resources,35(3), pp.1-2. Available at: https://dpi-proceedings.com/index.php/dtssehs/article/view/7637 [Accessed 20 Dec. 2017].

Marketing Mix Essay Analysis Essay Example

Marketing Mix Essay Analysis Essay The new event of procedure scheduled in the recent epoch is the launch of e-Business. This procedure is fundamentally an extra method for the growing of productiveness and an addition in the zone of net income for the concern that is involved. The cardinal construction of the e-Business is the active public-service corporation of the computing machines and on-line minutess. This besides involves assorted schemes to finish the procedure and manage the construction with nucleus techniques for the best consequence. The acquired procedure of selling and direction in the recent old ages have grown and taken its topographic point with latest techniques and new acronyms in the planetary touch. We will write a custom essay sample on Marketing Mix Essay Analysis specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Marketing Mix Essay Analysis specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Marketing Mix Essay Analysis specifically for you FOR ONLY $16.38 $13.9/page Hire Writer E-Business is the new integrating of the activities of the administration or the company and their merchandises along with their services and processs in the cyberspace. A old traditional concern linked with the new growing of enables e-Business scheme incorporating the activities of the administration or the company having gross revenues, accounting, selling and many more operations that are concerned with the organisation and their procedure involved takes us to the pride of accomplishing the hereafter growing and their enlargement. Outline Section: This assignment includes the necessary of placing the rules of selling and a complete analysis of the construction of e-business in to the chosen illustration. The clip to clip market explains the merchandise launch and their association with the market for the release. It redirects the right clip for the consideration of launch of the undertaking or pattern in conformity with the execution alterations that has to take topographic point within the administration as a affair of fact. Stigmatization: The procedure of branding shall be concerned as the portion of beginnings provided in the cyberspace and other resources about the trade name and by the undertakings of concern of engagement. The web page usually supplies the relevant range of information about the trade name and the administration with its benefits and service provided on the existent. A website establishes the fact of the company or the organisation and creates consciousness about the offers and handiness of the strategies provided by the company. It besides brings the latest key on notice to the immediate range of the clients and creates a satisfaction among the clients. The procedure of branding non merely has an impact on the clients but besides enriches the beginning of clients with a physique of trust and raises itself with a value of accreditation in the market of competition. The trade name becomes a portion of the quality and promise of the clients and the spouses along with the employees as a portion of fl exibleness, trust and growing. Singapore Zoo: The Singapore Zoo, Located at 80 Mandai Lake Road, is besides known as Mandai Zoo or Singapore Zoological Garden. Popular for its Night Safari attractive force, the Zoo has a reaped a long list of awards, both local and international. This has to be the one of the truly beautiful menagerie, of which there are all excessively few. It is superb laid out, so that the ceclosure fencings are about wholly concealed. The sweeps of the H2O of the seletar reservoir are a brilliant extra characteristic of the scene. The animate beings are attractively maintained in good wellness, in malice of the climatic conditions. All in all, the Zoo shall have a warn felicitation for the caputs that are responsible for them, and declaring it for us was a great experience to see it. The treasure of an thought to hold a menagerie was conceived by Dr. Ong Swee Law. Opened on 27 June 1973, the Singapore Zoological gardens caters to recreational, educational and societal demands of Singaporeans and tourer to the democracy. The Open Zoo which reflects the absence of physical barriers in enclosure design. Alternatively cascading Waterss, exuberant flora and fosses smartly mask the concealed barriers to heighten the semblance of freedom and natural jungle-like ambiance for the animate beings. The Zoo began with the low aggregation of 270 animate beings of about 72 species. To day of the month, a sum of over 2000 animate beings of 240 species are displayed in some 70 exhibits. One of the more appealing attractive forces at the menagerie is the carnal show. The animate being shows Archpriest Reptile show s and Elephant and Sea-lion shows. Due to popularity and rush in crowds, an Amphitheatre was built in 1985 to centralise these shows and to sit visitants comfortably. The amphitheater which has a long pool with glass panels for aquatic Acts of the Apostless can sit 1,500 and can suit up to 2,000 witnesss. The Animal Shows are specially designed to integrate the natural behavior of the animate beings to educate visitants in an entertaining presentation. The preservation messages are disseminated to the audience during the shows to rise their consciousness of the environment. In 1987, the Zoo introduced the Particular loan exhibit, where rare animate beings on loan from other Zoos are displayed for a short period. The animate beings which have been displayed at the particular Loan Exhibit include the aureate monkeys, white Liberation Tigers of Tamil Eelams and elephantine coon bear. This fresh thought gives visitant s an chance to see rare animate beings which can non be exhibited for good at the menagerie. The enclosure was renamed Singaporean Press Holdings ( SPH ) enclosure following its acceptance by the SPH group in 1990. Another major discovery for the Zoo was the debut of submerged sing exhibits. The first was in 1988 the submerged sing polar bear exhibit enable visitants to r ub olfactory organs with the Zoo s polar bear. Since 1987, the Zoo passed the million Markss in visitant attending. The twine of awards bagged by the Singapore Zoo attests to splendid dwellers and services: 2002 Asean Tourism Association ( ASEANTA ) Awards for Excellence, Best New attractive force in ASEAN ( Singapore Zoo for Hamadrya s Baboons The great rift vale of Ethiopia ) . 2001: STB ( Singapore Tourism Board ) Tourism Awards: Leisure Attraction of the Year 2001 ( Singapore Zoo ) . 2001 The Singapore Totalisator Board Excellence Singapore Award: Internationally acclaimed unfastened menagerie design and first-class unmoved preservation accomplishment. 2000: Where Singapore magazine s Concierge Choice Awards: Best Topographic point to Take the Kids. 1999: IMA ( Incentive A ; Meeting Asia ) Awards: Best Subject Attraction. Important Campaigns in the procedure of integrating of the administration in e-Business are as follows ; A new architecture of scheme and launch of concern mechanization by the procedure of e-Business. An enhanced position and recommendation of the developed solution in the web and front-end bequest applications. A new format of the developed portals of the organisation or the company with the update of the latest information. New value concatenation integrated through the web based application for the company or the organisation. SWOT analysis: The SWOT analysis is an apprehension of the entire consequence of the current place of the concern. It defines the Strength, failing, chances and the menaces of the organisation fiting the external chances with the internal strengths. The company or the organisation affecting for the SWOT analysis has to order the schemes and strengths to keep in control the failings and extinguish the menaces in any from the external beginnings. Strengths: They are unfastened 365 yearss all around the twelvemonth and it is considered as a most amused tourer attractive force. They open at 8:30 am in the forenoon and stopping point at 6:00 autopsy in the eventide making an enthusiastic environment for all ages from 6 to 90 daily all around the twelvemonth. The last ticket sale everyday is available at 5:30 autopsy enabling the visitants to come in at the event of shutting for short clip enjoyments excessively. The cost of the entry is besides sensible for the attractive forces available in the subject wood, the Singapore Zoo. Rental of the saunterer is available enabling a hassle free travel until the Zoo and the charges are besides sensible as $ 9 merely. Complimentary admittance for DDR ( Disability Development Registry ) card holders and entitlement of beneficiary programmes chosen by the Voluntary Welfare associations. Protection and preservation comes with instruction. The visit enhances us to understand the necessary of preservation and protection raising consciousness. Organizing events such as nuptials and parties for ain ground with sensible cost vouching the success of the juncture conducted. Sponsorship and acceptance besides remains as a portion of the squad along with little contribution originating the success of protection and preservation of wildlife. The location suits the entry itself with a dark campaign and orchid gardens howling itself with pride for sing. Shows are alone and sing Australian outback, cat state, critters Longhouse, Fragile forest, Elephants of Asia, Hamadryas Baboons, Orang Utan, Polar Bear, Proboscis Monkeys, Primate Kingdom, Rainforest Kidzworld, Reptile Kingdom, Wild Africa, White tiger are the chief attractive forces. Failing: Weather: The conditions circumstance may be the stop point, as anticipation is neer accurately right hence the market anticipation refers consequently. Cost: This cost may sound sensible when sing the factors of the entire value and convenience referred in the topographic point. Topographic point: The location depends convenient on consideration upon the general factors but the alone ideas upon the each visitant vary. Shows: Visitors though they are happy with the shows and penchants that are given or organised by the administration but they prefer to hold more shows. Opportunities: The Singapore Zoo is said to be an organized subject with shows, events and attractive forces. They tend to hold increased in their growing along with the old ages. Now they have become a portion of the wildlife protection and preservation making consciousness in the societal media. There are rather a batch of chances and competition that exist in the field in and around the universe. Menaces: There are no immense menaces when concerned with the whole of the administration whereas some exist such as the competition between the other subjects around the country. There exist another dark campaign and orchid gardens for sing. The location Singapore itself refers itself for a composure and howling entertaining country. The visitant can go a patron or a member, he can affect himself as a portion of the squad enriching the lives of animate beings the admiration and an flight for the world in esteem. They are the beginnings of a life amusement and they have to be preserved. Marketing mix: The most of import elements of selling are defined as the 4 P s. They are Product, publicity, monetary value and topographic point. An organisation may alter the elements of marketing harmonizing to their demands and inclinations. The mark for their ain caste in the market is set with official appellation with the change of the rules of marketing mix if required and are taken in to consideration for the fact of gaining their benefits in the resource implemented. The chief constituent to be considered is the addressing of the competitory market and the execution and the procedure of support by the operations merged to turn out the range of the mark through the selling mix rules. Taking the selling mix in this case for this administration comprises of the merchandises, topographic point, publicity and distribution. The merchandises offered are the existent offering that is present in the location and the monetary value is the value that remains exchanged for the offering nowadays in the topographic point. The publicity includes the targeting of the audience with the mix of publicities for the attractive force of visitants. Distribution covers the topographic point where the merchandise is being offered and located for the targeting of the audience. Topographic point: The location is been chosen as the best suitable topographic point for the building of the Zoo and it s the perfect topographic point for the procedure chosen by the organisation. The organisation built has assorted thoughts such as scheduling events and marrying thoughts indoor or out-of-door events. They besides categorize themselves in organizing the events and catering and all beginnings for the engaged events. The location sets scenic position and manner for the other topographic points of attractive force such as the dark campaign and orchid gardens that cover the tourist musca volitanss. The major point discovers the country as a whole of tourer attractive force and determines itself with a assortment of attractive force such as dark campaign and gardens on manner to the Rainforest menagerie. They besides become a portion of other administrations such as the menagerie s and wildlife preservation within the state. The carnal brushs and drives, feeding times, carnal picture taking, menagerie store plays an of import function in the pride of being alone among the other attractive forces. Monetary value: The cost of the entry is effectual when considered the shows and characteristics of the Zoo. The monetary value is $ 20 per grownup and $ 13 for the Child aged within 6-12. Students grant and group allowances are available when booked as a whole and has to be contacted the organisation. They have some programs for the handicapped and authorized visitants leting a free entry for the DDR card holders. Merchandise: The menagerie comprises of assorted shows in exhibiting the wildlife of the administration and other specific animate beings such as White tiger, delicate forest, wild Africa, Elephants of Asia, cat state, Orang Utan, Reptile Garden, Polar bear, Proboscis Monkeys, Rainforest Kidzworld, Primate Kingdom, Hamadrya s Baboons, Critters Longhouse, Australian outback. It is besides considered as the finest menagerie in Asia and ranks top among the beautiful menagerie of the universe excessively. The animate beings adopt an unfastened construct of life in the landscape and spread across the vivid and broad environment separated for the funny witnesss with wet and dry fosses. The shows are of assortments and the flicker for enthusiasm prevarication in high potency for clients to return frequently in sing the menagerie. It is considered to be the universe s first menagerie with dark wild life park on may 26th 1994. It covers the 2nd topographic point for the dense wood divided among the E and west cringles. An extraordinary experience with the nocturnal species of more than one 1000 animate beings within a count of 100 species toggled in the technique of soft visible radiation of visibleness. The dark campaign is said to the wholesome and popular amusement in the dark pulling about all the visitants of tourer attractive forces present in the metropolis. Over the past decennary it has non let itself one per centum down with the freshness. It has loaded itself with four awards in the recent yesteryear including the local and international being a portion of the IFEA Pinnacle award of the 2001 with a present rubric as the Gold victor. The Night campaign procedure is besides celebrated for its Gourmet campaign Express. Promotion: The publicities of the administration comprises of the carnal picture taking, guided Tourss, Animal brushs and drives, feeding times, nutrients and drinks, stores in menagerie, friendly installations for the handicapped and shows organised harmonizing to the periods of season. Education and preservation are portion of the promotional strategy for the visitants to acquire involved with the societal infinite. The kids s universe was created in the twelvemonth of 1973 consisting of the drama land and the carnal land. The celebrated illumination train drive that connects the lands together and the animate beings are placed in such a mode that they exist in their natural home ground unlike the unreal influence and stimulation. The carnal land in entitled for the kids s to play and with a existent life experience as the domestic animate beings participate in the drama. The domestic animate beings include the poulets, coneies and sheep. The drama land is expeditiously placed with conventional equipments used for drama heightening the infinite of life. The kids s universe has been voted the best location for the childs in the magazine Where Singapore inaugural concierge pick 1999 awards. This is the first universe broad amusement publicity among the other specimens in the same class all around the universe to present the assortments of elements such as the underwater exhibits an chance to see the rare animate beings within the short span of clip as the menagerie is merely 37 old ages old. The menagerie is amphitheatre centralised that was built in the twelvemonth 1985 and now it has reached the highs of audience including the archpriest, reptilian and elephant shows get downing in the 1970 s and assisting the launch of amphitheater with more than 1500 to 2000 seats of adjustment. Awards: The awards have been contributed for the specialized services and glorious dwellers of the menagerie. The Zoo ab initio started with 270 animate beings of about 72 species and has grown to an extent of 2000 animate beings lending from the 240 species displayed in about 70 exhibits. The awards are both local and international as listed below ; 1999 Best theme Attraction- IMA ( Intensive and meetings Asia Awards ) 2000 Concierge pick awards Where Singapore Magazine Best topographic point for childs 2001 Leisure attractive force of the twelvemonth Singapore Totalisator Board of Excellence for Singapore award 2001 Singapore touristry Board ( STB ) Leisure attractive force the twelvemonth 2001. 2002 ASEANTA Asean Tourism Association Awards for excellence: Best New Attraction. Experiences: The assorted attractive forces that are found in the administration is said to be dimensional and are the best experience in one s life after the visit. The new vision of administrating and keeping the species of life illustration and their preservation is the most of import characteristic. The visitants all over the universe have ever been satisfied and this administration has taken the pride of supplying more than the cost involved per individual for the amusement. The events organised consist of graphic ceremonials personalised harmonizing to the engagement made by the person and general events such as nuptials and jubilations of specific occasions are organised in such a manner that they are most remembered and considered the best in the heads of famed single household. The huge figure of crowds and choice of shows organised in the menagerie harmonizing with the seasons play an of import function for the visit of assorted visitants and audiences. Selling Controls: Market research, client studies, engagement, sponsorship, attending with on-field success is considered to be the chief controls of marketing by and large. The e-business dramas an of import function in the selling functions for this administration. The web site is specially designed to lend al the efficiency and amusement lively examining the client to take part in the visit. The design of the website enhances the quality and administration of part in a specialised manner. It besides shows complete informations of the menagerie and farther to the facts present it besides allots a opportunity for the engagement of the visitant along with the exhilaration offers. Decision: The overall consideration and the above study show the complete focal point of the administration. The study after the scrutiny from the history until the latest mark of the administration sing the facts along with the advantages and disadvantages conclude that they are listed in the top ranking for the attractive forces world-wide. This consequence shows the specific activity and growing of their selling through a new median of e-Business establishing their complete attractive forces and consideration with a practical position over the available charts of the administration. The selling schemes that actively take part in the function of increasing their growing from the past decennaries have contributed much to their highs of sweetening. Hence the procedure of growing has taken an of import drive in the selling scheme and mix along with nucleus key of E-Business.

The Welfare State A Cost Benefit Analysis essays

The Welfare State A Cost Benefit Analysis essays The Welfare State - A Cost Benefit Analysis The role of welfare within our society has always been controversial. This problem emphasizes the need to understand the roles of variable factors when pertaining to the subject of welfare within our society. The proposed analysis will address the phenomenon of welfare assistance and several factors which may contribute to the increase or decrease of welfare assistance to the poor in 4 ways: (1) by defining major concepts and any other concepts about which there is likely to be misunderstanding (2) by further examining the past history pertaining to the subject of welfare assistance within the United States; (3) by developing the formulation of a hypothesis which will provide for an explanation of welfare; and finally (4) determining whether or not the benefits of welfare assistance outweigh the cost. Ultimately, the purpose of this research analysis is to investigate variable factors that may contribute to the increase or decrease of welfare assistance. This cost benefit analysis is an attempt to explain the tentative assumptions of others pertaining to the subject of welfare, in order to determine and explain the relationship of welfare to the economic cost and benefits. Before welfare assistance can be analyzed there is a need to define the terms that will be used. Policies like welfare assistance are worthwhile only if the benefits to society are greater than the costs. When choosing among a set of policies, the policy with the greatest net benefit (benefit over cost) should be chosen. Hence, this is where the term cost-benefit analysis comes from. Cost-benefit analysis is a technique for determining the optimal level of an economic activity such as welfare. In general, an activity such as welfare assistance should be expanded as long as it leads to greater benefits than costs. In purely economic terms, does the benefit of welfare assistance justify the costs of welf...

Reglas para el ingreso de mascotas a Estados Unidos

Reglas para el ingreso de mascotas a Estados Unidos El ingreso de mascotas est sujeto a reglas que hay que seguir. Tener en cuenta que aquà ­ se explican las que afectan a las aduanas estadounidenses pero que hay que respetar tambià ©n las del otro paà ­s del que se viaja o que es destino final. Lo primero que hay que saber es que las reglas aplican tanto a las personas que residen habitualmente en Estados Unidos y regresan al paà ­s con su mascota como a los que viven en otros paà ­ses e ingresan a los Estados Unidos por la razà ³n que sea. Los segundo, es que las mascotas deben de estar sanas. Y si no lo estn pueden ser sometidas a cuarentena. Y que en casos especà ­ficos simplemente se prohibir su ingreso. Y lo tercero es que hay reglas especà ­ficas segà ºn el tipo de la mascota. Asimismo,  si se considera que est en peligro aplicarn reglas especiales. A continuacià ³n los casos ms comunes de reglas para mascotas y hacia el final la normativa que regula el ingreso de las piezas de caza en Estados Unidos. Se acaba con un listado de los puertos de entrada al paà ­s para el caso de caza y mascotas especiales. Reglas para ingreso de roedores a Estados Unidos Conejos, conejillos de indias, hamsters y hurones que son mascotas pueden ingresar a Estados Unidos si tienen buena salud. Si no la tuvieran, sern sometidos a cuarentena y el dueà ±o deber correr con los gastos. Los erizos sà ³lo se pueden traer a travà ©s de determinados puertos de entrada, que incluye aeropuertos. Consultar con la CBP. En todo caso, no se pueden ingresar los que proceden de Nueva Zelanda.   En cuanto a ratas, puerco espà ­n, ratones, ardillas y lirones, estn prohibidos sà ³lo si proceden de Africa.   Reglas para ingresar con perros a Estados Unidos Deben tener un certificado de vacuna contra la rabia si proceden de un paà ­s en las que existe tal enfermedad. Adems, se requiere uno de que est libre de gusano barrenador (screwworm en inglà ©s), si procede de un paà ­s en el que existe dicha enfermedad. Finalmente, si se trata de un perro guà ­a y se ingresa a los Estados Unidos en avià ³n deber consultarse con la aerolà ­nea del vuelo, por si piden documentacià ³n adicional.   Reglas para viajar con gatos a Estados Unidos No se requiere la presentacià ³n de la vacuna contra la rabia en el caso de animales que son mascotas.   Reglas para ingresar a mascotas como monos y dems primates No pueden ser traà ­dos a Estados Unidos como mascotas en ninguna circunstancia. No se admiten excepciones. Ingreso a Estados Unidos de tortugas como mascotas Las tortugas vivas con caparazà ³n y que miden ms de 4 pulgadas y que se ingresan como mascotas pueden ser importadas sin restricciones. Pero siempre a travà ©s de un puerto de entrada que se ocupa de tramitar la papelerà ­a para animales salvajes (ver ms abajo). Las tortugas vivas con caparazà ³n y menos de 4 pulgadas pueden ser importadas sin fines comerciales, pero con un mximo de 7 ejemplares. Lo mismo sucede con los huevos viables de tortuga. Tambià ©n deben de ingresar a travà ©s de un puerto de entrada que procesa animales salvajes.   Peces Si no estn incluidos en listas de animales en peligro de extincià ³n ni en listados de especies daà ±inas (injurios species) entonces pueden ser importados como mascotas. Es necesario utilizar para ello uno de los puertos de ingreso designados para tramitar la entrada de peces  al paà ­s Reglas que aplican a la caza para ingresar a Estados Unidos Las reglas para ingresar a los Estados Unidos aves y otros animales abatidos por cazadores son como siguen: En primer lugar, si se trata de un pjaro o de otro animal no considerado como protegido y que ha sido cazado por un un ciudadano estadounidense o por un residente permanente legal en Canad o en Mà ©xico y el fin de la importacià ³n es no comercial: se puede producir el ingreso en cualquier puerto de entrada (aeropuerto, puerto o frontera terrestre). Pero es necesario llenar la planilla Form 3-177. En segundo lugar, si la pieza cazada procede de otro paà ­s entonces el ingreso se debe realizar a travà ©s de un puerto designado para tramitar esa documentacià ³n. Adems, deber presentarse papeles mostrando la licencia del cazador, sellos y el documento de exportacià ³n del paà ­s en el que se haya cazado al animal, si dicho paà ­s lo requiere. Finalmente, tener en cuenta que para no pagar impuestos de importacià ³n es preciso que la pieza haya sido legalmente cazada por el que la trae, es decir, no puede ser comprada. Asimismo, es necesario ser ciudadano o residente permanente de los Estados Unidos.   Aves migratorias, animales protegidos o considerados amenazas Si se ha cazado un ave migratoria o se desea importar un animal o mamà ­feros marinos o cualquier otro animal considerado protegido o amenazante para las especies de los Estados Unidos, consultar antes con el Servicio de Peces y Fauna, porque podrà ­a estar prohibido traer la pieza a los Estados Unidos. El telà ©fono al que marcar es el 1-800-358-2104. Ahà ­ se puede obtenre informacià ³n sobre quà © documentacià ³n adicional se podrà ­a necesitar o si, simplemente, no es posible traer un determinado animal a los Estados Unidos.   Atencià ³n a los ingresos de marfil El paso de marfil por las Aduanas de los Estados Unidos est sujeta a restricciones, incluso cuando forma parte de instrumentos o muebles. Puerto de entrada para procesar la papelerà ­a de Peces y Fauna El Servicio de Peces y Fauna (Fish and Wildlife en inglà ©s) est presente en los siguientes puertos de entrada: AtlantaBaltimoreBostonChicagoDallas / Fort WorthHonoluluLos Angeles  MiamiNueva OrleansNueva YorkNewarkPorlandSan Francisco  Seattle A tener en cuenta para pasar control migratorio y aduanero fcilmente Para evitar tener problemas en las aduanas, esto es un compendio de todo lo que no se puede ingresar o est sujeto a algà ºn tipo de restricciones, desde alimentos a medicamentos.   Adems del control migratorio, al llegar a un puerto de entrada a Estados Unidos se pasa por un control migratorio. Esto es lo que saben los oficiales fronterizos. Finalmente, cabe destacar que si el ingreso se realiza a travà ©s de uno de los puestos de control migratorio en la frontera entre Mà ©xico y EE.UU. las demoras pueden ser largas, particularmente en fechas seà ±aladas. Para evitarlo o estar preparado es posible consultar cunto tiempo se est tardando en cruzar en cualquier puesto en particular. Este es un artà ­culo informativo. No es asesorà ­a legal.

Econometrics 'A' project for MSc Economics degree course Essay

Econometrics 'A' project for MSc Economics degree course - Essay Example However, in this research we are only interested in examining the effect of total experience and attainment of a specific level of education on the wage differential between the sexes. Total experience was obtained by adding ‘months since hire’ and ‘previous Experience’ This study utilized secondary sources for data collection. The use of secondary data is useful because it is cost efficient and saves time for the independent researcher (Glaser, 1963; Hyman, 1978; Hayashi, 2000). The independent researcher has limited financial resources because he or she works alone. Such researchers are usually professionals who attempt to research some significant social problem on a part-time basis. Description of the Research Design A two-step attempt was made to discover if the income of professional females increases at the same rate as that of males with comparable education as the experience increase. During the first step the existence and strength of a relationshi p between salary and experience for male and female professionals with a given university degree in a specific job were researched using correlational methodology. During the second step a comparison was made between the incomes of males and females with the same experience. Attainment of an educational degree was held constant for each given professional position. The correlational research method was selected as the primary methodology because of its ability to discover relationships among logical variables (Wooldridge, 2008). Research Hypothesis It appears that there is a lack of congruence in the literature in establishing an association between level of income, experience and gender, and also a lack of significant research efforts towards establishing a link between income and experience by gender identification, therefore, the hypothesis was stated as a null hypothesis (Albrect et al., 2003; Atkinson et al., 2003; Blau et al., 2006). Locke, Spirduso, and Silverman (1987) recom mend that if one researches a question where no well-established theory exists one should use the format of the null-hypothesis. There will be no difference in the salary of males and females at given experience when other factors, such as academic degree (bachelor's, master's, or associate degree), and professional position are held constant. Variables The variables in this study's regression analysis are as follows: Male/female relationship (the numerical value of 0 was assigned to the male and 1 to the female variable), education and experience were considered indepen ­dent variables; annual salary depends on the aforementioned variables and therefore is considered the dependent vari ­able. A dummy variable was introduced by multiplying experience by the value of the male/female relationship for the reason mentioned below. The first independent variable, that is, male/female, was selected for its possible effect on the difference in earnings between the sexes. The second inde pendent variable, experience (in months), was chosen for its possible effect on the earning of any individual. The third indepen ­dent variable, education, allows the individual to enter a given professional position. And finally the fourth independent variable, the dummy variable, was supposed to trigger possible changes over time, that is, the closing of the male/female

The e-businesss decision making and the customers decision makings Literature review

The e-businesss decision making and the customers decision makings effect each other - Literature review Example Assael believed that the process of decision-making can mainly divided into two parts, choosing and making decision (P48, 2000). It is a well known that the decision making process is an essential aspect of the business and most leaders are aware of the intensity and importance of decision making to the business. The process of decision making generally involves a high number of uncertainties and confusions. As very well explained by Chris Brogan, â€Å"Things are running well and you come up with a hundred new ideas to expand; Things go poorly, and you find yourself grinding through dozens of ideas of what will fix it. But the success youll eventually experience will come from understanding those decisions deeply† (Brogan, 2010). The main aim of good decision making is the need to understand the decisions and choices being made. Decision making has been referred to with a number of words like ‘difficult, perplexing, and nerve racking’ (Arsham, 2010). It is essential to note that these terms are only used on decision making as the process of making a decision is one which is a critical moment for the business and can impact the business in a number of ways. Decisions are the heart and blood of organizations and need to be taken with extreme care and with high levels of focus. Decision making has been defined as â€Å"the process of responding to a problem by searching for and selecting a solution or course of action that will create value for organizational stakeholders† (Scribd, 2010). It is also essential to note that decision making stands on six C’s, these include, a) Construct, b) Compile, c) Collect, d) Compare, e) Consider, f) Commit. Having understood the meaning of decision making, it is essential to understand the decision making process. The next sub section details the decision making process. The decision making process is more of looped process and

Managing Culture and Change Essay Example for Free

Managing Culture and Change Essay The present scenario has made the future of organizations uncertain. Companies that are excelling in their relevant industries right now may be pushed out in the near future by upstarts with better ideas and more versatility than them. It has happened so often in the past that the only certainty is that it will keep on happening in the future. The only long term sustainable competitive advantage that organizations can hope to create is through their people, and the behaviour of these people is largely determined by the corporate culture in which they are immersed. A successful corporate culture will see the organization through the near future, but unless the organization is proactive in its bid to keep on adapting to the changing business environment, that same corporate culture may sound the death knell for the organization in the future. CORPORATE CULTURE AND PERFORMANCE The question on every entrepreneur, every CEO, and on every member of the Board of Directors’ mind since before the inception of the concept of an organization as an institution has been: how to make more money? Once this fundamental urge has been recognized one can comprehend the reason behind the actions of the above-mentioned in ensuring the success of their respective organizations, as the evidence of past decades have proved that organizational success is the key determinant of greater financial reward for them; hence stemmed the need to identify the factors that would translate into success for an organization. The idea that an organizations culture would influence its performance is hardly new. ‘Search of Excellence’, published in 1982 and authored by Waterman and Peters made the concept famous, and in 1992 in ‘Corporate Culture and Performance’, Hesket and Kotter compared various organizations and the relation of their performance within a time period with their cultures. But with increasing research being conducted on the correlation between an organizations vision and the employees’ actions, doubt is cast on the efficacy of expecting that an organizations culture will determine its performance. Instead, consensus is being established that a few core values largely determine the success or failure of organizations. SUCCESS Success of an organization can be defined differently depending on the industry in which the organization operates. An organization in a high risk industry can view success as the ability to survive all these years. Another organization may view continuing growth over and above competitors as success, while still another might seek to become the market leader in its particular industry in order for it to be considered a success. Pyramid of Organizational Development Whatever the perception an organization has, in order to be successful an organization needs to follow six steps or tasks at each level of its growth in order for it to succeed at all of those stages. It needs to determine its market niche in which it will operate, develop the product/service accordingly, acquire the relevant resources be they land labour or capital, develops a system of standardized procedures for operations, and then the same for management, and finally, develop a corporate culture that management perceives as being important to lead the firm. Before any further discussion can be carried out, corporate culture must be defined as, a structure of collective values and beliefs that work together with the systems, the organizational structure, and the employees to produce norms, which then turn into what is necessary to the organization, and the processes of getting things done – the how and the why. It is an intangible aspect of an organization that cannot be seen, touched, heard or smelt, but is present nonetheless and which ultimately determines whether that organization will succeed or not. It is the core values that were mentioned earlier, to produce the norms that determine the behaviour of the employees, which ultimately determines the productivity and thus success of the firm. The steps taken together are framed as a hierarchical model into a pyramid of organizational development that emphasizes the importance of each step for the organizations development depending on the stage of growth the organization is in at that point. The last three of the steps form the structure that will be the sustainable competitive advantage of the firm, inimitable by competitors, thus ensuring long term success for the firm. ROLES OF CORPORATE CULTURE Corporate culture by itself plays several roles in the organization. It develops a cultural identity in an employee by establishing a commitment of the employee to achieving the organizations goals and objectives. That identity serves to increase the devotion and involvement of the employee by making him/her reconcile the organizations success as his/her own. Corporate culture also uses a deductive approach to align an employee’s behaviour with acceptable norms that have been already determined by the organization so that it need not worry about policing the employee to do what is right according to the organization. Instead of the management having to interfere now the employee’s peers can bring a maverick back in line with acceptable behaviour as determined by the values of the organization. In addition to these, an organizations culture acts as a motivational tool that is more effective than managerial insistence. However, if the organization’s stated values are out of sync with the culture that is being promoted the employees will become disillusioned with the organization and its stated commitment to quality of work life as the rewards will be based on the stated values as opposed to what the prevailing organizational culture promotes. TYPES OF CORPORATE CULTURE Strong The desired roles that the culture of the organization is to play and reality may starkly differ depending on the type of culture that is prevalent in the organization. A Strong Culture is reminiscent of an organization that spends a significant amount of time and resources in communication and reinforcement by management of the values of the organization to the various employees. This is facilitated by having well-defined vision and mission statements that have been established with the help of both the top and bottom rung of the organization. In addition, these statements and their espoused values are adopted by top management and thus succeed in their task of influencing the employees by convincing them of their commitment to the organization and its value system. Weak On the other hand a Weak Culture is quite the opposite, in its lack of cultural identity and the divergence of employee behaviour and organizational goals. A weak culture also involuntarily imparts an image of punishing those employees that might be working towards organizational success, through the actions of their peers who have this weak corporate value system ingrained into them. Thus there is little allegiance to the organization and little unity inside it. Unhealthy An Unhealthy corporate culture rests on the basis that change is undesirable. In such an environment risk-taking and experimentation for the sake of innovation is frowned upon, and so the organization has to seek ideas outside the firm for better practices and approaches. This is a costly result of the politicized environment that prevails in such an organization, where decisions are made based on the power wielded by the participants and not on the value of their respective input. Adaptive The fourth type of culture in an organization is an Adaptive one which is contrary to the unhealthy culture in its regard for entrepreneurial activities. Such an organization is a proactive entity that actively seeks out innovative and experimental solutions to any issues that might have to be faced. Risk taking is encouraged to the extent that employees might be funded to bring their ideas to life, in the recognition that successful adaption to the environment through risky enterprises enable the organization to prosper over its competitors. CONFLICT IN CORPORATE CULTURE An organizations culture is influenced by the social power of relationships, more specifically, the potential loss of those relationships. This invokes cognitive dissonance in a person more than does any other action, and thus motivates us the most to make sure that such a situation does not arise. From this motivation an organizations culture is developed as a means to protect these relationships, leading to the theory of conflict resolution and culture development. Social influences in the form of formal and informal networks, family networks, relationships with leaders, and legal regulations are what determine the behaviour of a person in an organization with regards to conflict resolution. Although conflict invariably leads to closer bond in relationships than before, it can also lead to rejection or coercion by one or both party. The conflict can only be resolved when both parties either cooperate in or reject the relationship. The eventual conflict resolution results in a differing viewpoint held by the onlookers as either a matter of public discourse or of private thought, which viewpoints are shared with others depending on the closeness of their relationship. And while cooperative conflict resolutions foster greater trust and unity, competitive conflict resolution in hierarchically dominated organizations, where management often passes judgement rather than mediates, results in a chasm between formal and informal cultures that causes a drop in organizational performance. HUMAN RESOURCE MANAGERS Human Resource Management has become an increasingly important function in organizations with the findings of several researches supporting the relation between high-performance and HR practices. The recognition through these researches that the only long term sustainable competitive advantage that an organization enjoys is the capabilities of its human resource has further caused organizations to reinvent this previously neglected function as well as resulted in an increase in the perceived advantages of a strategically-focused Human Resource Department. Amongst these, cultural management is an important benefit that pertains to the development, communication, reinforcement, and change of the corporate culture to the employees. SUCCESSFUL CORPORATE CULTURE An organization’s corporate culture can either hinder or contribute to the success of a firm by influencing the behaviours and attitudes of employees with respect to the overall organizational strategy. A corporate culture that promotes behaviours that are aligned with the company’s values provides guidance to the employees with respect to the accepted norms of the organization and reinforces those peer actions that support the desired culture. The HR as the manager of the corporate culture aligns its own practices in an effort to increase commitment amongst employees towards achieving the desired vision of the organization by reaching their performance targets, and by engaging in adaptive and innovative entrepreneurial activities. A successful corporate culture is one which supports the organizations execution of its strategy, with the help of HR managers, who align the desired values of the organization with the values that are actually prevalent. While some organizations seek to prescribe specific corporate values that will lead to greater performance, it has been discovered that instead it is the fit of these values to the internal and external realities of the organization – the employees’ behaviour and the business environment – that the HR must focus on as a culturally compatible management system. To do so, the practices of the HR Management, more specifically, the staffing, training and development, incentive and payment packages, performance appraisals and job design, all become areas where HR can influence the cultural communication strategy. As a company that dedicated its existence to the production and sale of coffee, Starbucks has experience phenomenal growth to reach its current market leadership position. From generating sales of $122 million in 1993, Starbuck’s has grown to such an extent that its competitors have less outlets in all of the United States than Starbucks has in California. And the stated reason by its CEO, Howard Schultz, for this success is its people. â€Å"The way we treat our people affects the way they treat our customers and, in turn, our financial performance. † In lieu of this statement Starbucks offers good quality of work life to its employees, adopts diversity as an integral part of doing business, maintains a high standard in its production processes, develops its customers, engages in Corporate Social Responsibility, and recognizes the importance of maintaining profitability as a means of continued success. NOT-SO-SUCCESSFUL CORPORATE CULTURE Unfortunately, discovering the right fit of values that an organization can use as a component of its successful corporate strategy can become ‘the winner’s curse’ if left unmonitored, for while it is difficult to change any culture in any organization, changing a seemingly successful culture is next to impossible. No one wants to mess with a winning formula, whether due to superstition, the belief that it will weather any competition, or due to inability to accept that the environment has changed. Whatever the reason, history is replete with examples of how successful companies were brought to their knees by a more versatile competitor. The US automobile industry was indifferent of the lower-costing, higher-quality Japanese cars until it was too late, believing that the Japanese cars were just a momentary novelty. In the same way, until sales started decreasing fast Sears remained ignorant of the changes brought to the organizations environment by Wal-Mart. The McDonnell Douglas Corporation is another example of a company that failed to adapt to the changing environment, and was acquired by Boeing. Core Rigidities Inducting the use of Standard Operating Procedures as a means to increase production by cutting down on wastage of time and resources, while beneficial in producing economies of scale can be harmful to the future success of the firm as well. These procedures and routines can serve as a source of suitable solutions to any problems that might be faced, but in doing so negate the possibility of searching for knowledge and innovation outside the organization. Consequently the core competency of the firm is transformed into the core rigidity as exploration is shelved for exploitation. In addition to which, the feedback from the use of SOPs is ignored due to selective filter of already existing procedures which siphon out any corrective measures that the feedback might have been trying to highlight. This is largely due to the fact that no firm can keep changing forever. There is an absorptive capacity for every organization beyond which it is incapable of adapting to any more change. Crisis The only way to break out of such a potentially hazardous ‘advantage’ is for there to be an existence of a crisis that jolts the organization out of its routine. Some companies even try to introduce the perception of a crisis in the organization in order to stimulate innovation, such as Hewlett Packard, following the story by Sturgeon, ‘Microcosmic God’ in which Sturgeon talks of a miniature world which is introduced to numerous difficulties such as earthquakes, and droughts, and whose inhabitants are forced to either evolve or die out. While such practices might seem unethical it is disputed whether it is more advisable to let a firm fail because the leader of the organization could not bring it upon himself/herself to fool the employees for their future benefit. CONCLUSION If an organization’s successful culture eventually ends up costing the organization in the future, can it really be deemed a ‘successful’ corporate culture? Up to the limit of an organization’s absorptive capacity, a successful corporate culture should be redefined as a culture that while maintains all the characteristics as mentioned previously, is also a culture that can recognize that no matter what the fit of the organization’s culture is at present, that fit can change, and the possibility of that change should not be ignored, rather it should be actively sought out. And while it is a difficult thing to change a culture at any point, the organization should be ready to do so if it is to maintain its competitive advantage and not be replaced by a more nimble and recent competitor like Sears was by Wal-Mart. BIBLIOGRAPHY Cummings, T. G. Worley, C. G. (2005) Organization Development and Change. USA. Thomson South-Western. Davenport, T. H. Prusak, L. (2000) Working Knowledge. Harvard Business Press Hassard, J. Pym, D.(1993) The Theory and Philosophy of Organizations. Routledge Hitt, M. A. , Hoskisson, R. E. Ireland, R. D. (1998) Strategic Management: Competitiveness and Globalization. Cengage Learning Kono, T. Clegg, S. (1998) Transformations of Corporate Culture. Walter de Gruyter Kotter, J. (1992) Corporate Culture and Performance. Free Press. Thompson, A. A. Jr. , Strickland III, A. J. Gamble, J. E. (2006) Crafting and Executing Strategy. United States. McGraw-Hill Balkaran, L. (1995) Corporate Culture. Find Articles [Internet]. Available from http://findarticles. com/p/articles/mi_m4153/is_/ai_17365781 [December 19, 2008] Denison, D. How your corporate culture can impact your business performance. IMD Webletter [Internet]. Available from http://www01. imd. ch/webletter/pdf/LinkingCorporateCulture. pdf [December 19, 2008] Flamholtz, E. Hua, W. (2002) Strategic Organizational Development and the Bottom

Essay --

Introduction of Clinical Question In a normal brain neurons in the substantia nigra produce a chemical called dopamine that acts to control motor functions. In a person with Parkinson’s disease, there is insufficient dopamine and motor symptoms such as tremors, rigidity, problems with balance and bradykinesia can occur. According to the National Parkinson Foundation, four to six million people worldwide are estimated to have Parkinson’s Disease. In the United States alone, the incidence is estimated at fifty to sixty thousand new cases diagnosed each year. Additionally, the Center for Disease Control puts complications from Parkinson’s disease as the 14th leading cause of death. While there is no cure, the symptoms can be controlled with medications and surgery. [6] A 2011 German cross sectional study revealed that people with Parkinson’s Disease had poorer oral health. They were found to have more caries, tooth mobility, gingival recession and periodontal pocketing than the control cohort. Additionally, the group with Parkinson’s disease reported less frequent tooth brushing and dental visits, and exhibited reduced salivary flow. [4]. Supporting this, a separate Japanese study showed more untreated caries and deeper periodontal pockets, leading to fewer natural teeth in persons with Parkinson’s Disease [2]. There is a significant population with Parkinson’s Disease, a condition that has been shown to have a negative impact on oral health. Increased caries and periodontal problems can lead to tooth loss and edentulism, a condition treatable with traditional or implant retained overdentures. Patient RM is a male with Parkinson’s disease. He is partially edentulous with six maxillary teeth and two mandibular teeth. ... ...100. [2] Hanaoka A, Kashihara K. Increased frequencies of caries, periodontal disease and tooth loss in patients with Parkinson’s disease. Journal of Clinical Neuroscience. 2009; 16: 1279-1282. [3] Heckmann SM, Heckmann JG, Weber H-P. Clinical outcomes of three Parkinson’s disease patients treated with mandibular implant ovedentures. Clin Oral Impl Res. 2000; 11: 566-571. [4] Muller T, Palluch R, Jackowski J. Caries and periodontal disease in patients with Parkinson’s disease. Spec Care Dentist. 2011; 31(5): 178-181. [5] Packer M, Nikitin V, Coward T, Davis D, Fiske J. The potential benefits of dental implants on the oral health quality of life of people with Parkinson’s disease. Gerodontology. 2009; 26: 11-18. [6] Welcome to Parkinson.org [Internet]. Miami (FL): National Parkinson Foundation; 2014 [cited 2014 Mar 7]. Available from: http://www.parkinson.org/.

Cell and Molecular Biology

Cell and Molecular Biology Final Name: Hadeel Binomar30 pts. Protein misfolding can be an aspect of several different human disorders, including cystic fibrosis, Alzheimer's disease, and atherosclerosis. Many times, the misfolded protein is a membrane protein. In fact, a type of diabetes insipidus results from a mutation in the G-protein-coupled vasopressin 2 receptor that prevents the protein from making it to the cell membrane A) Describe how this type of receptor would normally get targeted to the membrane (from the beginning of translation) and B) then propose one mechanism by which the mutation could cause a lack of proper targeting. One of the most important protein's target is G protein-coupled receptors, several signaling mechanisms depend on this type of receptor to change both internal and external stimuli to the intracellular responses. Basically, one of the G couple receptor subfamilies is G-coupled Vasopressin-2- Receptor (V2R), and this receptor is going through a strict quality control process at the endoplasmic reticulum, which presents the only correctly folded protein to gets through the secretory pathway. The primary function of the V2 receptor is to activate the attached G protein that bound to the ? subunit then phosphorylated to GTP. The G protein couple receptor then activates the enzyme adenylate cyclase that catalyzes the reaction in the ER and forms cAMP from ATP. After that, cAMP acts as a second messenger and activates a protein kinase that phosphorylates the integral membrane proteins on the cell surface. Moreover, the secretory pathway organelles' and the plasma membrane both are first introduced into the Endoplasmic reticulum, and the co-translationally proteins that can cross the ER are synthesized by the ribosome first then binds by chaperones to gets moves to the ER surface using GTP that allows them to move toward the receptor then release it. The soluble proteins and the integral membrane proteins as I mentioned above, can be targeted through the ER and then translocated by the same mechanism.Further, several mutations occurring in the transmembrane region which affect the structure of the protein. These are multiple mutation sites, such as mutations occurring in the amino acid residues which was acting as a causative agent for human disease. Also, there are other mutations occurring on the single site position that will affect the translation mechanism and cause many human diseases like: cystic fibrosis, Alzheimer's disease, and atherosclerosis that proves the function of V2R protein which plays important role in the translation during the protein folding process. One of the mechanism that may a reason for lacking the proper target protein is when the mutations of the CFTR gene occurring and affect the function of the chloride ion channels and cause defect in the protein sequences which lead to the production of diseases and misfolded of the proteins that are unable to recognize their functional destinations. Otherwise, Lack of the stop signals is another issue that prevents the protein from getting into the surface of the ER, also called the non-stop decay cellular pathway, because lack of this stop signals prevents mRNA from synthesis and translate the proteins, these consider as a point mutation that inhibits the essential stop codons. 30 pts. Describe the experiment shown in figure 3 from the paper we discussed in class (Miller et al, 2003). (A) In your description, consider the following questions: Why did they do it? How did they do it? What did they learn? (B) Diagram the results that you would expect to see in Lanes T, 1, 2, 3, and 4, if the amino acid signal DID in the protein Gap1p was mutated to random amino acids and tell why; and (C) Give two possible (different) results that might occur if the amino acid signal LxxLE in Bet1p was mutated to the amino acids DID (which are the signal in Gap1p). Diagram the results expected in Lanes T, 1, 2, 3, and 4 and explain why you predicted this result for each case.left20840701.A001.A4467225201739500The experiment was performed to study the role of cargo binding domain of Sec24p in the process of protein sorting. To perform this study, both mutant and wild types subunit Sec23/24p and Sec23/24L616W were harvested from microsomal membranes. The immunoblotting assay performed to quantify cargo molecules using radio-labeled secondary antibodies. Comparison of the mutant subunit with wild type illustrated omission of some molecules in mutant one, these molecules were cargo protein molecules. While some of them are packed in a way that similar to the wild-type. It meant that there were some signals that remained unaffected although mutation was there. Unexpectedly, it was found that in these unaffected molecules of mutation in Sec23/24p, packaging was better than the wild type. Further, they found the proteins that were highly affected had Bet1p and Gap1p/Sys1p chimera, because they completely depending on the Sys1p di-acidic of COPII vesicles. If the amino acid signal DID in the protein Gap1p was mutated to random amino acids, the resulting bands would be seen as in figure 1. A because the amino acids might be present in all the lanes. And Gap1p is required for di-acidic motifs to fuse together with the COPII vesicles at the mutated domain. Also, Sys1 peptide is not involved in Sec 23/24p hence its mutation is not contained in a di-acidic motif. If the signal LxxLE was mutated to the DID, the expected Bet1p packaging would be shown in figure 2. A as not be seen in lanes containing Bet1p because the amino acid signal LxxLE does not exit after mutating to amino acids DID. The second probability for the Bet1p would be expected to bind with the vesicle for packaging when it interacts with di-acidic motifs which is needed as shown in figure 2.B290512515621000581025113030004781550412752.B002.B6286501346202.A002.A20 pts. Explain the experiment shown in Figure 5C from the Shen et al. (2018) paper on the phosphorylation of CDC25 that we discussed in class. In addition, be sure to also address the following: A) why did they do this experiment (the central issue); B) what did they learn; and C) what is another control experiment they could have done? D) Would these results change if you added a constitutively active form of LKB1 to the reaction? If so, how and why? A) Overall, the main purpose of this paper is to explain how the division of cells is highly regulated such that cells that fail to pass some specific stage-based tests cannot advance to the proceeding stages. In this particular experiment, HeLa cells were subjected to chemicals that are known to activate the enzyme AMPK. Also, the main catabolic processes that are involved to generate energy for cells to transition from G1/G2 were determined by the application of radiochemical approaches, the experiment required to approve how highly conserved cellular energy sensor can significantly delay mitosis entry and activation of AMP-activated protein kinase (AMPK). Further, Wee 1 family inactivates the cell cycle G2/M Phase which is controlled by Cdc3/cyclic B (mitotic cyclin-dependent kinase complex). They found that AMPK-dependents phosphorylation of CDC25C arranges a metabolic control point for the M-phase transition and the cell cycle phase G2. B) Also, they learned that suppression of Wee 1 or acute induction partially reinstates mitosis ingress in the circumstance of activated protein kinase (AMPK). This experiment showed that when Phosphorylates Cdc25 is in a distributive and disordered state, it results into ultra-sensitivity in protein phosphorylation. C) For another control experiment, they might try exposing the U2OS (cell line) clones conditionally exhibiting Cdc25A etoposide. That will help to test whether Cdc25A degradation is significant for the G2 control point or not.D) If they add an active form of LKB1 to the reaction, the results will not change, because in cells LKBI activity inhibits AMPK activation in response to different stimulations. Also, LKBI is lost upon consistent isolation and therefore no effects of phosphates observed.20 pts. Briefly describe the role of cyclin-CDK in the cell cycle and give an example of positive and negative control in this system. Also, describe how cell cycle regulation links to the stimulation of apoptosis at the molecular level. In the beginning, the cell cycle depends on many basic factors that control the regulation process starting from the signal transcription molecules, growth factors and the Cyclin Dependent Kinases enzymes include the checkpoints which control the transition process between the cell cycle phases by binding to the cyclin proteins CDKs then phosphorylate other proteins to transfer from one phase to another. The role of transcription factors is to turn on the signals for gene expression, DNA replication, and cell divisions. As an example of CDKs, cyclin-dependent kinase 1 CDK1 is a cell division cycle protein homolog 2, that has a primary role in human cancer cells because CDK1 rather than any types of CDKs is fatal to the mutated version of MYC- dependent cancer that leads to a depletion of oncogenes like (Fos and Jun) in human cancer cells. Fos and Jun are combined forms of the transcription factor called AP1 that activates the delayed response genes such as cyclin D and CDK4. Recent studies reveal that the reason for the MYC breast cancer cells duplation is targeting CDF1 exhibit any other CDKs cell lines. Also, CDK1 inhibition can control and target the cancer cells in human and both phosphorylation and expression of MYC during the cell cycle process. To initiate intracellular signaling pathways and stimulate the cell cycle entry, mitogens substance bind to the cell surface receptor with the activation form of GTPase Ras that activates MAP kinase cascade. That will lead to the expression of encoding gene of the transcription regulatory protein like MYC. Moreover, E2F transcription factor is a target for cyclin D and CDKs that stimulate proteins expression to initiate S phase, also E2F regulated by the tumor suppressor gene Rb (Retinoblastoma protein). At early G1 Phase, Rb protein combined with E2f to form the histone deacetylases protein that remains chromatic condensed, then cyclin kinase phosphorylates Rb protein, causing it to disassociate from E2F. That allows recruitment of histone acetylates, which decondense the chromatin and helps transcription complexes to form in G1 and S phase and to form a positive feedback. For the negative control, if there are no growth factors present to stimulate the synthesis of Cyclin D in the new cell, Rb phosphorylated will turn off, and Rb will rebind to E2F, then the cell cycle will stop and that will lead to many negative results like prevent it to bind with DNA, or DNA damage and cells will return to the G0 phase. Apoptosis is a consequence of DNA damage; if the damage is not repaired, the cell cycle will divert towards apoptosis, otherwise, if the cell has DNA defects and fail to undergo apoptosis, that will change to the cancer cell. G1 phase regulators such as P53 and E2F are essential to promote the cell regulations and eliminate any damage or abnormal changes during the cell cycle. P53 has a primary function to prevent any mutation in DNA during cell progression, so any defect in P53 will lead to cancer, and it's usually mutated in cancer cells. Further, Rb protein considers as a tumor suppressor and also promotes apoptosis. Additionally, most human cancers have inactive Rb protein, either mutated Rb or the non-phosphorylated (inactivated form) of Rb protein